
MASCIR - Postdoctoral Researcher in Cell Biology and Protein Purification
- بن جرير
- عقد
- دوام كامل
- Establishing and optimizing cell culture protocols under sterile conditions (adherent or suspension eukaryotic cell lines).
- Designing, establishing protocols and conducting recombinant protein expression experiments using eukaryotic or prokaryotic systems (HEK293, CHO, E. coli, etc.).
- Applying and optimizing transfection methods (chemical, electroporation) or viral infection techniques (adenovirus, baculovirus).
- Purifying target proteins using chromatographic techniques (affinity, ion exchange, gel filtration).
- Analyzing and characterizing purified proteins using SDS-PAGE, Western blot, ELISA, UV spectroscopy, BCA/Bradford assays, etc.
- Actively contributing to critical data analysis, protocol optimization, and the writing of scientific reports.
- Supporting the valorization of research outcomes through scientific publications and/or patent applications.
- Supervising students and interns involved in the project.
- Strong expertise in sterile cell culture techniques (eukaryotic cell lines).
- Proven experience in recombinant protein expression (transient and/or stable expression systems).
- Strong expertise in molecular biology techniques (nucleic acid extraction, PCR, RT-PCR, etc.).
- Proven experience in protein purification methods (FPLC/HPLC chromatography).
- Strong expertise in protein analysis tools (SDS-PAGE, Western blot, ELISA, UV spectroscopy, BCA/Bradford assays).
- Experience in animal experimentation would be an asset.
- Scientific autonomy and strong experimental rigor.
- Strong organizational skills.
- Critical thinking and problem-solving abilities.
- Ability to work effectively in a multidisciplinary team.
- Good communication skills in both English and French (oral and written).
Times Higher Education